Killing of T Lymphocytes By UFT/ACNO/ShenQU
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Take 10ml blood, dilute in 10ml RPMI.
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Add into 50ml conical tube containing of 18 ml Ficoll-pague(pharmacia) gently on the top(do not mix)
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Centrifuge at 2,000 rpm, 30min at room temperature.
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Pool cells from the interphase area with Ficoll(cloudy area), dispense these cells in new 50ml tube.
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Centrifuge at 2,000 rpm, 15min, 4oC. Cells are not packed tightly. Discard supernatant with pipette.
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Resuspend in RPMI. Centrifuge at 2,000, 10min, 4oC.
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Wash twice with 20ml RPMI, Centrifuge at 2,000 rpm, 15min, 4oC.
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Resuspend in 10ml of complete medium with 10% FCS(RPMI + FCS+ Penicillin/strep+Glu) for growing cells.
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Counting cells and dilute to a count of 4 x 106 T cells/ml in Complete Medium.
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Take 1ml cells into each well of 24-well plate, add 20ulUFT, or ACNO, or ShenQu, transfer into CO2 incubator overnight at 37oC.
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Counting the killing of cells.
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Results: T cells death%
Volume UFT* ACNO** ShenQU*** Medium****
20ul 95.8-96.7% 11.3-14.7% 3.7-5.6% 99.5-100%
*UFT one kind chemotherapy.
** ACNO Chinese medicine for anticancer
*** ShenQu negative Chinese herb
**** Medium positive control group.
T lymphocytes belong to the human immune system and play first line against cancer. However when the patients take chemotherapy ,the cells are also killed . The results showed that chemotherapy and medium control group almost kill all normal T-lymphcytes. However the Chinese medicine ACNO and ShenQu have a very low influence on the T cells. That means that the Chinese medicine kills the cancer cells and does not damage the normal cells.